Combining genetic, biochemical, and structural approaches, the authors show how translation of rpsU (bS21) in Flavobacterium johnsoniae is autoregulated by ribosomes lacking bS21. The mechanism requires release of the anti–Shine–Dalgarno (ASD) sequence of 16S rRNA from a binding pocket on the 30S platform domain, formed in part by bS21. This work also uncovers a role of the ASD in 30S subunit biogenesis.

Articles:

Role of the anti-Shine–Dalgarno sequence of 16S rRNA in Flavobacterium johnsoniae | Nucleic Acids Research | Oxford Academic

Structure of a specialized 70S initiation complex | Nucleic Acids Research | Oxford Academic